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World’s smallest dog cloned 49 times


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“Miracle Milly” holds the title of the world’s smallest dog. The six-year-old Chihuahua is only 9.5 centimetres tall.

But that’s not the only record she holds – she’s also the most cloned dog, being recreated a whopping 49 times.

Owner Vanessa Semler, 38, from Florida reached out to the Sooam Biotech Research Foundation in Seoul, Korea to find out more about Milly’s genetic structure and why she is so small.

The miniscule pup weighed only 28 grams when she was born. She was so small her entire body could fit in the head of a teaspoon and there were concerns she would not survive.

“The original idea was to make 10 clones in total, nine for research and one for us to keep. But they decided to clone her more times,” Ms Semler told Caters News Agency.

“They wanted to find out why she was so small and then study her genes to find out what makes her so tiny.”

Pet cloning doesn’t come cheap, at more than $100,000 per clone. But it allows grieving pet owners to recreate their perfect pet.

The Sooam lab was established in 2006 and now clone hundreds of dogs each year. The cloning technique they use is the same method used on Dolly the sheep in 1996.

First cells were taken from Milly to be cloned externally, then the nucleus, which contains her genetic information, was removed and saved.

Milly’s nucleus was inserted into a donor egg cell which was then given a small electric shock to stimulate cell division.

Once that succeeded, the developing embryo was inserted into a surrogate mother. The surrogate doesn’t have to be a Chihuahua but must be small enough to provide a similar environment for the pregnancy to succeed.

Ms Semler currently lives with 12 of Milly’s clones. Their names are Molly, Mally, Melly, Molly II, Mumu, Mila, Mary, Mimi, Moni, Mini, Mela and Mulan.

“It’s amazing to be around all of her clones. They are so smart, very playful like Milly and have very similar personalities,” Ms Semler said.

The research data collected from Milly’s cloning journey will be analysed and published in a scientific journal.

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